The following was taped on Thursday in Orlando, Florida at Universal Studios.
3/17 TNA One Night Only Knockouts PPV taping:
* Maria and Mike Bennett opened the show cutting a promo about the Queen of the Knockouts 2016 providing an opportunity to eight “aspiring Knockouts” to eight current Knockouts on the roster. Each winner advances to a battle royal.
* The eight aspiring Knockouts were Allysin Kay, Shelly Martinez, Deonna Purazzo, Leva Bates, Barbie Hayden, Cherry Bomb, Veda Scott and Jayme Jameson.
* Allysin Kay def. Gail Kim via countout.
* Rebel def. Shelly Martinez.
* Laura Dennis def. Velvet Sky.
* Jade def. Leva Bates.
* Rosemary def. Veda Scott.
* Barbie Hayden def. Raquel.
* Marti Belle def. Jayme Jameson.
* Madison Rayne def. Deonna Purazzo.
* Maria & Mike Bennett def. Gail Kim & DJ Z.
* Jade eliminated Madison Rayne to win the battle royal to become Queen of the Knockouts.
* Xplosion: Rockstar Spud def. Robbie E.
* Jessie Godderz def. Crazzy Steve.